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rabbit anti cd86  (Proteintech)


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    Structured Review

    Proteintech rabbit anti cd86
    Rabbit Anti Cd86, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 578 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cd86/CD86+Antibody/pm42914281-48-0-10
    Average 96 stars, based on 578 article reviews
    rabbit anti cd86 - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: RUVBL2 Regulates Microglia Metabolic Reprogramming to Mediate Stress Granules Aggregation Exacerbating Postoperative Delirium in Aged Mild Cognitive Impairment Rats.
    Article Snippet: .. The membranes were incubated overnight at 4°C with primary antibodies: anti- RUVBL2 (1:1000, Catalog Number: ab19027, Abcam, Cambridge, UK), antiα- Ketoglutarate Dehydrogenase (OGDH) (1:1000, Catalog Number: 15212- 1- AP, Proteintech Group Inc., Wuhan, China), anti- Pyruvate Kinase 2 (PKM2) (1:1000, Catalog Number: 15822- 1- AP, Proteintech Group Inc., Wuhan, China), anti- CD86 (1:1000, Catalog Number: 13395- 1- AP, Proteintech Group Inc., Wuhan, China), anti- Arginase 1 (Arg1) (1:1000, Catalog Number: 16001- 1- AP, Proteintech Group Inc., Wuhan, China), and anti- Tubulin (1:50,000, Catalog Number: 10094- 1- AP, Proteintech Group Inc., Wuhan, China) as the internal reference. .. After three washes with Tris- buffered saline containing Tween- 20 (TBST), the membranes were incubated with the appropriate secondary antibodies at room temperature for 1 h. Following additional washing, the PVDF membranes were exposed to enhanced chemiluminescence (ECL) reagent (Elabscience Biotechnology Co. Ltd., Wuhan, China) for visualization and imaging.

    Article Title: Cu 2+ -chelated epigallocatechin gallate nanoparticle-functionalized hydrogel promotes osteogenesis by inhibiting ferroptosis.
    Article Snippet: A pathological inflammatory microenvironment can hinder bone regeneration by disrupting the osteogenic differentiation potential of bone marrow-derived mesenchymal stem cells (BMSCs).. However, conventional antiinflammatory therapies alone are insufficient to fully resolve this challenge.. Bone defect repair is often impaired by persistent inflammation-induced ferroptosis in BMSCs, and traditional approaches fail to prevent lipid peroxidation-driven cell death.

    Article Title: Lactate Promotes an Anti-Inflammatory Phenotype in Activated Microglia
    Article Snippet: Cells were rinsed with 1 mL of 1X PBS and fixed with 4% paraformaldehyde (PFA) (15670799, Fisher Scientific) for 20 min at room temperature, then rinsed again with 1X PBS. .. After permeabilization of cell membranes in PBS buffer containing 0.25% (v/v) Triton X-100 for 15 min, blocking was performed using 2% (w/v) BSA buffer with 0.25% (v/v) Triton X-100 for 1 h. Cells were incubated overnight at 4 °C with primary antibodies: anti-Iba1 (Abcam, ab48004) to confirm microglial identity, and anti-GFAP (Proteintech, 16825-1-AP) to exclude astrocytic contamination, and anti-CD86 (Proteintech, 13395-1-AP) or anti-CD206 (Abcam, ab64693) to identify pro-inflammatory and anti-inflammatory phenotypes, respectively. .. Following three washes with 1X PBS containing 0.05% Triton X-100, appropriate secondary antibodies (Alexa Fluor 488 or 568, Abcam) were applied for 1 h. Coverslips were mounted with Vectashield containing DAPI (4′, 6-diamidino-2-phenylindole, Vector Laboratories) and imaged using a Nikon Eclipse 90i microscope (20× and 40×).

    Western Blot:

    Article Title: Chaihu-Jia-Longgu-Muli Tang ameliorates chronic restraint stress-induced depressive-like behaviors in association with modulation of the gut microbiota-NLRP3 inflammasome-microglia axis.
    Article Snippet: 1 Academy of Chinese Medical Sciences, Henan University of Chinese Medicine, 156 Jinshui East Road, Zhengdong New District, Zhengzhou 450046, P.R.. China 2 Collaborative Innovation Center of Research and Development on the Whole Industry Chain of Yu-Yao in Henan Province, Henan University of Chinese Medicine, Zhengzhou 450046, P.R.. China Abstract Chronic stress-induced neuroinflammation and gut microbiota dysbiosis have been implicated in the pathogenesis of depression.

    Immunofluorescence:

    Article Title: Pomegranate-inspired bionic microgel/periosteum composite harnessing reactive oxygen species for immune-engineered bone regeneration.
    Article Snippet: Excessive reactive oxygen species (ROS) and unresolved inflammation create a hostile microenvironment that compromises regeneration in critical-sized bone defects.. Here, we developed a pomegranate-inspired bionic microgel/periosteum composite (PBC) featuring a hierarchical “membrane–seed” modular design, in which a periosteum-mimetic PDA/PLLA membrane provided not only ROS counteraction but also a continuous protective interface and defect compartmentalization.. Conductive GelMA/PEDOT:PSS microgels serving as the “seeds” enabled enhanced and prolonged ROS clearance as well as conformal filling of irregular geometries, and increased cell–material contact.

    Staining:

    Article Title: Pomegranate-inspired bionic microgel/periosteum composite harnessing reactive oxygen species for immune-engineered bone regeneration.
    Article Snippet: Excessive reactive oxygen species (ROS) and unresolved inflammation create a hostile microenvironment that compromises regeneration in critical-sized bone defects.. Here, we developed a pomegranate-inspired bionic microgel/periosteum composite (PBC) featuring a hierarchical “membrane–seed” modular design, in which a periosteum-mimetic PDA/PLLA membrane provided not only ROS counteraction but also a continuous protective interface and defect compartmentalization.. Conductive GelMA/PEDOT:PSS microgels serving as the “seeds” enabled enhanced and prolonged ROS clearance as well as conformal filling of irregular geometries, and increased cell–material contact.

    Article Title: Mapping intestinal microenvironment heterogeneity in Hirschsprung's disease by spatial transcriptomics: Role of THBS1 in macrophage polarization and neural stem cell regulation
    Article Snippet: .. A tatal of 1 × 10 6 THP-1 cells were collected from each treated group, washed with phosphate-buffered saline, and stained with fluorochrome-conjugated anti-CD206 (Proteintech, FITC-98031) and anti-CD86 (Proteintech, FITC-98025) according to the manufacturer's instructions. .. Flow cytometry was performed on a FACScan system (BD Biosciences), and the data were analyzed with FlowJo software.

    Article Title: Bioactive Platinum Nanozymes Accelerate Diabetic Wound Healing via Anti-Inflammation and Macrophage Polarization Modulation
    Article Snippet: .. Main materials including sodium hyaluronate (SHA) (from Streptococcus Equi, 95% purity, the molecular weight of 1,350,000–1,650,000 Da; Macklin Biochemical Tech), sodium borohydride (NaBH 4 ; Sinopharm Chemical Reagent), chloroplatinic acid (H 2 PtCl 6 ; Aladdin Chemistry), anti-IL-4, anti-IL-1β, anti-CD31, anti-α-SMA and anti-TGF-β1 (Thermo Fisher Scientific), Hematoxylin and Eosin (H&E) staining kit, Masson staining kit and DPAI (Beyotime), anti-CD68, anti-CD206 and anti-CD86 (Proteintech) were used. ..

    Saline:

    Article Title: Mapping intestinal microenvironment heterogeneity in Hirschsprung's disease by spatial transcriptomics: Role of THBS1 in macrophage polarization and neural stem cell regulation
    Article Snippet: .. A tatal of 1 × 10 6 THP-1 cells were collected from each treated group, washed with phosphate-buffered saline, and stained with fluorochrome-conjugated anti-CD206 (Proteintech, FITC-98031) and anti-CD86 (Proteintech, FITC-98025) according to the manufacturer's instructions. .. Flow cytometry was performed on a FACScan system (BD Biosciences), and the data were analyzed with FlowJo software.

    Blocking Assay:

    Article Title: Lactate Promotes an Anti-Inflammatory Phenotype in Activated Microglia
    Article Snippet: Cells were rinsed with 1 mL of 1X PBS and fixed with 4% paraformaldehyde (PFA) (15670799, Fisher Scientific) for 20 min at room temperature, then rinsed again with 1X PBS. .. After permeabilization of cell membranes in PBS buffer containing 0.25% (v/v) Triton X-100 for 15 min, blocking was performed using 2% (w/v) BSA buffer with 0.25% (v/v) Triton X-100 for 1 h. Cells were incubated overnight at 4 °C with primary antibodies: anti-Iba1 (Abcam, ab48004) to confirm microglial identity, and anti-GFAP (Proteintech, 16825-1-AP) to exclude astrocytic contamination, and anti-CD86 (Proteintech, 13395-1-AP) or anti-CD206 (Abcam, ab64693) to identify pro-inflammatory and anti-inflammatory phenotypes, respectively. .. Following three washes with 1X PBS containing 0.05% Triton X-100, appropriate secondary antibodies (Alexa Fluor 488 or 568, Abcam) were applied for 1 h. Coverslips were mounted with Vectashield containing DAPI (4′, 6-diamidino-2-phenylindole, Vector Laboratories) and imaged using a Nikon Eclipse 90i microscope (20× and 40×).

    Molecular Weight:

    Article Title: Bioactive Platinum Nanozymes Accelerate Diabetic Wound Healing via Anti-Inflammation and Macrophage Polarization Modulation
    Article Snippet: .. Main materials including sodium hyaluronate (SHA) (from Streptococcus Equi, 95% purity, the molecular weight of 1,350,000–1,650,000 Da; Macklin Biochemical Tech), sodium borohydride (NaBH 4 ; Sinopharm Chemical Reagent), chloroplatinic acid (H 2 PtCl 6 ; Aladdin Chemistry), anti-IL-4, anti-IL-1β, anti-CD31, anti-α-SMA and anti-TGF-β1 (Thermo Fisher Scientific), Hematoxylin and Eosin (H&E) staining kit, Masson staining kit and DPAI (Beyotime), anti-CD68, anti-CD206 and anti-CD86 (Proteintech) were used. ..



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    Image Search Results


    EZH2 inhibition reduced the inflammatory phenotype of macrophages during OA. Macrophage differentiation and M1-like polarization were induced from the THP-1 monocytic cell line ( a ) or from human bone marrow-derived cells isolated from OA patients ( b ) in the presence or absence of EPZ-6438. The expression of pro-inflammatory markers (IL-1β, IL-6 and CXCL1) was analyzed by RT-qPCR. Experiments using THP-1 cells were independently repeated at least three times. Experiments using human bone marrow-derived cells were performed using biological replicates from independent OA patients. Data are presented as mean ± SEM. *: p -value < 0.05; **: p -value < 0.01; ***: p -value < 0.001. Spleens from OA mice described in Fig.  and treated with vehicle or EPZ-6438 were collected and analyzed by flow cytometry. Cells were stained for macrophage-associated markers ( c ). Samples were acquired using a FACSVerse flow cytometer and analyzed with FlowJo 7.6.5 software. Results are presented as median ± interquartile range (n = 8 mice). *: p -value < 0.05; **: p -value < 0.01

    Journal: Molecular Biomedicine

    Article Title: EZH2 coordinates inflammatory and pain-associated pathways across joint compartments in osteoarthritis

    doi: 10.1186/s43556-026-00643-w

    Figure Lengend Snippet: EZH2 inhibition reduced the inflammatory phenotype of macrophages during OA. Macrophage differentiation and M1-like polarization were induced from the THP-1 monocytic cell line ( a ) or from human bone marrow-derived cells isolated from OA patients ( b ) in the presence or absence of EPZ-6438. The expression of pro-inflammatory markers (IL-1β, IL-6 and CXCL1) was analyzed by RT-qPCR. Experiments using THP-1 cells were independently repeated at least three times. Experiments using human bone marrow-derived cells were performed using biological replicates from independent OA patients. Data are presented as mean ± SEM. *: p -value < 0.05; **: p -value < 0.01; ***: p -value < 0.001. Spleens from OA mice described in Fig. and treated with vehicle or EPZ-6438 were collected and analyzed by flow cytometry. Cells were stained for macrophage-associated markers ( c ). Samples were acquired using a FACSVerse flow cytometer and analyzed with FlowJo 7.6.5 software. Results are presented as median ± interquartile range (n = 8 mice). *: p -value < 0.05; **: p -value < 0.01

    Article Snippet: The following antibodies were used for immune cell characterization: CD11b (REA592 VioBlue, 130–113–810, Miltenyi Biotec), F4/80 (REA126 PE-Vio770, 130–118–459, Miltenyi Biotec), CD80 (REA983 APC-Vio770, 130–116–463, Miltenyi Biotec), CD86 (REA1190 PE, 130–122–129, Miltenyi Biotec), MHC-II (REA813 FITC, 130–112–386, Miltenyi Biotec), CD38 (REA616 PerCP-Vio700, 130–109–260, Miltenyi Biotec), and iNOS (REA982 APC, 130–116–423, Miltenyi Biotec).

    Techniques: Inhibition, Derivative Assay, Isolation, Expressing, Quantitative RT-PCR, Flow Cytometry, Staining, Software