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mouse anti cd86 antibody  (Proteintech)


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    Structured Review

    Proteintech mouse anti cd86 antibody
    Representative double immunofluorescence images of <t>CD86</t> (red) and CD206 (green) (A) magnification: ×400. Quantification of relative fluorescence intensity of CD86 (B) and CD206 (C) Relative fluorescence intensity was calculated as the ratio of the MFI of each sample to the mean MFI of the Con group. MFI: mean fluorescence intensity. * P < 0.05 compared with the Con group.
    Mouse Anti Cd86 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 561 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cd86/CD86+Antibody/pmc13658527-98-17-21
    Average 96 stars, based on 561 article reviews
    mouse anti cd86 antibody - by Bioz Stars, 2026-10
    96/100 stars

    Images

    1) Product Images from "Trans-cuff budesonide delivery and its effects on endotracheal intubation-induced subglottic injury"

    Article Title: Trans-cuff budesonide delivery and its effects on endotracheal intubation-induced subglottic injury

    Journal: Frontiers in Pediatrics

    doi: 10.3389/fped.2026.1886065

    Representative double immunofluorescence images of CD86 (red) and CD206 (green) (A) magnification: ×400. Quantification of relative fluorescence intensity of CD86 (B) and CD206 (C) Relative fluorescence intensity was calculated as the ratio of the MFI of each sample to the mean MFI of the Con group. MFI: mean fluorescence intensity. * P < 0.05 compared with the Con group.
    Figure Legend Snippet: Representative double immunofluorescence images of CD86 (red) and CD206 (green) (A) magnification: ×400. Quantification of relative fluorescence intensity of CD86 (B) and CD206 (C) Relative fluorescence intensity was calculated as the ratio of the MFI of each sample to the mean MFI of the Con group. MFI: mean fluorescence intensity. * P < 0.05 compared with the Con group.

    Techniques Used: Immunofluorescence, Fluorescence

    Related Articles

    Immunofluorescence:

    Article Title: Icariin alleviates corneal neovascularization by regulating inflammation through inhibition of macrophage polarization and the NF-κB pathway
    Article Snippet: Icariin was purchased from TargetMol (Fig. A; 489–32 − 7, purity 97.64%). .. Antibodies for immunofluorescence were anti-VEGFA (Proteintech, 81323-2-RR), anti-CD86 (Affinity, DF6332), and anti-CD206 (Proteintech, 60143-1-Ig). ..

    Article Title: Pomegranate-inspired bionic microgel/periosteum composite harnessing reactive oxygen species for immune-engineered bone regeneration.
    Article Snippet: Excessive reactive oxygen species (ROS) and unresolved inflammation create a hostile microenvironment that compromises regeneration in critical-sized bone defects.. Here, we developed a pomegranate-inspired bionic microgel/periosteum composite (PBC) featuring a hierarchical “membrane–seed” modular design, in which a periosteum-mimetic PDA/PLLA membrane provided not only ROS counteraction but also a continuous protective interface and defect compartmentalization.. Conductive GelMA/PEDOT:PSS microgels serving as the “seeds” enabled enhanced and prolonged ROS clearance as well as conformal filling of irregular geometries, and increased cell–material contact.

    Incubation:

    Article Title: RUVBL2 Regulates Microglia Metabolic Reprogramming to Mediate Stress Granules Aggregation Exacerbating Postoperative Delirium in Aged Mild Cognitive Impairment Rats.
    Article Snippet: .. The membranes were incubated overnight at 4°C with primary antibodies: anti- RUVBL2 (1:1000, Catalog Number: ab19027, Abcam, Cambridge, UK), antiα- Ketoglutarate Dehydrogenase (OGDH) (1:1000, Catalog Number: 15212- 1- AP, Proteintech Group Inc., Wuhan, China), anti- Pyruvate Kinase 2 (PKM2) (1:1000, Catalog Number: 15822- 1- AP, Proteintech Group Inc., Wuhan, China), anti- CD86 (1:1000, Catalog Number: 13395- 1- AP, Proteintech Group Inc., Wuhan, China), anti- Arginase 1 (Arg1) (1:1000, Catalog Number: 16001- 1- AP, Proteintech Group Inc., Wuhan, China), and anti- Tubulin (1:50,000, Catalog Number: 10094- 1- AP, Proteintech Group Inc., Wuhan, China) as the internal reference. .. After three washes with Tris- buffered saline containing Tween- 20 (TBST), the membranes were incubated with the appropriate secondary antibodies at room temperature for 1 h. Following additional washing, the PVDF membranes were exposed to enhanced chemiluminescence (ECL) reagent (Elabscience Biotechnology Co. Ltd., Wuhan, China) for visualization and imaging.

    Article Title: Cu 2+ -chelated epigallocatechin gallate nanoparticle-functionalized hydrogel promotes osteogenesis by inhibiting ferroptosis.
    Article Snippet: A pathological inflammatory microenvironment can hinder bone regeneration by disrupting the osteogenic differentiation potential of bone marrow-derived mesenchymal stem cells (BMSCs).. However, conventional antiinflammatory therapies alone are insufficient to fully resolve this challenge.. Bone defect repair is often impaired by persistent inflammation-induced ferroptosis in BMSCs, and traditional approaches fail to prevent lipid peroxidation-driven cell death.

    Article Title: USP18 improves mitochondrial homeostasis by stabilizing PKM2 and promoting M2 polarization in macrophages to relieve acute lung injury.
    Article Snippet: Background: Acute lung injury (ALI) has high incidence and mortality rates among patients.. Studies have shown that USP18 is widely involved in the immunomodulatory process and that macrophage polarization plays a key role in the progression of ALI.. This study aimed to explore the potential molecular mechanism through which USP18 promotes M2 macrophage polarization and alleviates ALI.

    Staining:

    Article Title: Pomegranate-inspired bionic microgel/periosteum composite harnessing reactive oxygen species for immune-engineered bone regeneration.
    Article Snippet: Excessive reactive oxygen species (ROS) and unresolved inflammation create a hostile microenvironment that compromises regeneration in critical-sized bone defects.. Here, we developed a pomegranate-inspired bionic microgel/periosteum composite (PBC) featuring a hierarchical “membrane–seed” modular design, in which a periosteum-mimetic PDA/PLLA membrane provided not only ROS counteraction but also a continuous protective interface and defect compartmentalization.. Conductive GelMA/PEDOT:PSS microgels serving as the “seeds” enabled enhanced and prolonged ROS clearance as well as conformal filling of irregular geometries, and increased cell–material contact.

    Article Title: Bioactive Platinum Nanozymes Accelerate Diabetic Wound Healing via Anti-Inflammation and Macrophage Polarization Modulation
    Article Snippet: .. Main materials including sodium hyaluronate (SHA) (from Streptococcus Equi, 95% purity, the molecular weight of 1,350,000–1,650,000 Da; Macklin Biochemical Tech), sodium borohydride (NaBH 4 ; Sinopharm Chemical Reagent), chloroplatinic acid (H 2 PtCl 6 ; Aladdin Chemistry), anti-IL-4, anti-IL-1β, anti-CD31, anti-α-SMA and anti-TGF-β1 (Thermo Fisher Scientific), Hematoxylin and Eosin (H&E) staining kit, Masson staining kit and DPAI (Beyotime), anti-CD68, anti-CD206 and anti-CD86 (Proteintech) were used. ..

    Molecular Weight:

    Article Title: Bioactive Platinum Nanozymes Accelerate Diabetic Wound Healing via Anti-Inflammation and Macrophage Polarization Modulation
    Article Snippet: .. Main materials including sodium hyaluronate (SHA) (from Streptococcus Equi, 95% purity, the molecular weight of 1,350,000–1,650,000 Da; Macklin Biochemical Tech), sodium borohydride (NaBH 4 ; Sinopharm Chemical Reagent), chloroplatinic acid (H 2 PtCl 6 ; Aladdin Chemistry), anti-IL-4, anti-IL-1β, anti-CD31, anti-α-SMA and anti-TGF-β1 (Thermo Fisher Scientific), Hematoxylin and Eosin (H&E) staining kit, Masson staining kit and DPAI (Beyotime), anti-CD68, anti-CD206 and anti-CD86 (Proteintech) were used. ..



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    Effect of DID on macrophage polarization in tendon‐bone healing mice. (A) Representative images from flow cytometric analysis of F4/80 + <t>CD86</t> + ; (B) quantitative analysis of F4/80 + CD86 + ; (C) representative images from flow cytometric analysis of F4/80 + CD206 + ; (D) quantitative analysis of F4/80 + CD206 + ; (E) levels of IL‐1β mRNA as measured by qRT‐PCR; (F) levels of IL‐6 mRNA as measured by qRT‐PCR; (G) levels of Arg1 mRNA as measured by qRT‐PCR; (H) levels of IL‐10 mRNA as measured by qRT‐PCR. Data are expressed as the mean ± SD ( n = 3 biological replicates). * p < 0.05, ** p < 0.01 vs. sham group; # p < 0.05, ## p < 0.01 vs. model group.
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    Effect of DID on macrophage polarization in tendon‐bone healing mice. (A) Representative images from flow cytometric analysis of F4/80 + <t>CD86</t> + ; (B) quantitative analysis of F4/80 + CD86 + ; (C) representative images from flow cytometric analysis of F4/80 + CD206 + ; (D) quantitative analysis of F4/80 + CD206 + ; (E) levels of IL‐1β mRNA as measured by qRT‐PCR; (F) levels of IL‐6 mRNA as measured by qRT‐PCR; (G) levels of Arg1 mRNA as measured by qRT‐PCR; (H) levels of IL‐10 mRNA as measured by qRT‐PCR. Data are expressed as the mean ± SD ( n = 3 biological replicates). * p < 0.05, ** p < 0.01 vs. sham group; # p < 0.05, ## p < 0.01 vs. model group.
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    Effect of DID on macrophage polarization in tendon‐bone healing mice. (A) Representative images from flow cytometric analysis of F4/80 + <t>CD86</t> + ; (B) quantitative analysis of F4/80 + CD86 + ; (C) representative images from flow cytometric analysis of F4/80 + CD206 + ; (D) quantitative analysis of F4/80 + CD206 + ; (E) levels of IL‐1β mRNA as measured by qRT‐PCR; (F) levels of IL‐6 mRNA as measured by qRT‐PCR; (G) levels of Arg1 mRNA as measured by qRT‐PCR; (H) levels of IL‐10 mRNA as measured by qRT‐PCR. Data are expressed as the mean ± SD ( n = 3 biological replicates). * p < 0.05, ** p < 0.01 vs. sham group; # p < 0.05, ## p < 0.01 vs. model group.
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    Effect of DID on macrophage polarization in tendon‐bone healing mice. (A) Representative images from flow cytometric analysis of F4/80 + <t>CD86</t> + ; (B) quantitative analysis of F4/80 + CD86 + ; (C) representative images from flow cytometric analysis of F4/80 + CD206 + ; (D) quantitative analysis of F4/80 + CD206 + ; (E) levels of IL‐1β mRNA as measured by qRT‐PCR; (F) levels of IL‐6 mRNA as measured by qRT‐PCR; (G) levels of Arg1 mRNA as measured by qRT‐PCR; (H) levels of IL‐10 mRNA as measured by qRT‐PCR. Data are expressed as the mean ± SD ( n = 3 biological replicates). * p < 0.05, ** p < 0.01 vs. sham group; # p < 0.05, ## p < 0.01 vs. model group.
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    Effect of DID on macrophage polarization in tendon‐bone healing mice. (A) Representative images from flow cytometric analysis of F4/80 + <t>CD86</t> + ; (B) quantitative analysis of F4/80 + CD86 + ; (C) representative images from flow cytometric analysis of F4/80 + CD206 + ; (D) quantitative analysis of F4/80 + CD206 + ; (E) levels of IL‐1β mRNA as measured by qRT‐PCR; (F) levels of IL‐6 mRNA as measured by qRT‐PCR; (G) levels of Arg1 mRNA as measured by qRT‐PCR; (H) levels of IL‐10 mRNA as measured by qRT‐PCR. Data are expressed as the mean ± SD ( n = 3 biological replicates). * p < 0.05, ** p < 0.01 vs. sham group; # p < 0.05, ## p < 0.01 vs. model group.
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    CD73‐overexpressing ADSCs regulate macrophage polarization toward the M2 and inhibit NLRP3 inflammasome activation. (A) Flow cytometry results showing macrophage polarization in each group. <t>CD86</t> was detected using FITC‐conjugated antibody, and CD163 was detected using PE‐conjugated antibody. (B) Western blot and quantitative analysis of NLRP3, ASC, and caspase‐1 expression in vitro. (C) Quantitative analysis of Western blot. (D) ELISA analysis of IL‐1β and IL‐18 levels in each group in vitro. Statistical significance is indicated as ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001. All experiments were independently performed in triplicate. The full‐length, uncropped Western blot images are provided in Figure . ELISA data are summarized in Tables and .
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    Representative double immunofluorescence images of <t>CD86</t> (red) and CD206 (green) (A) magnification: ×400. Quantification of relative fluorescence intensity of CD86 (B) and CD206 (C) Relative fluorescence intensity was calculated as the ratio of the MFI of each sample to the mean MFI of the Con group. MFI: mean fluorescence intensity. * P < 0.05 compared with the Con group.
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    Representative double immunofluorescence images of <t>CD86</t> (red) and CD206 (green) (A) magnification: ×400. Quantification of relative fluorescence intensity of CD86 (B) and CD206 (C) Relative fluorescence intensity was calculated as the ratio of the MFI of each sample to the mean MFI of the Con group. MFI: mean fluorescence intensity. * P < 0.05 compared with the Con group.
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    Image Search Results


    Effect of DID on macrophage polarization in tendon‐bone healing mice. (A) Representative images from flow cytometric analysis of F4/80 + CD86 + ; (B) quantitative analysis of F4/80 + CD86 + ; (C) representative images from flow cytometric analysis of F4/80 + CD206 + ; (D) quantitative analysis of F4/80 + CD206 + ; (E) levels of IL‐1β mRNA as measured by qRT‐PCR; (F) levels of IL‐6 mRNA as measured by qRT‐PCR; (G) levels of Arg1 mRNA as measured by qRT‐PCR; (H) levels of IL‐10 mRNA as measured by qRT‐PCR. Data are expressed as the mean ± SD ( n = 3 biological replicates). * p < 0.05, ** p < 0.01 vs. sham group; # p < 0.05, ## p < 0.01 vs. model group.

    Journal: Immunity, Inflammation and Disease

    Article Title: Didymin may Enhance Tendon‐Bone Healing Partly by Promoting PPAR‐Mediated Fatty Acid Oxidation and Macrophage M2 Polarization

    doi: 10.1002/iid3.70485

    Figure Lengend Snippet: Effect of DID on macrophage polarization in tendon‐bone healing mice. (A) Representative images from flow cytometric analysis of F4/80 + CD86 + ; (B) quantitative analysis of F4/80 + CD86 + ; (C) representative images from flow cytometric analysis of F4/80 + CD206 + ; (D) quantitative analysis of F4/80 + CD206 + ; (E) levels of IL‐1β mRNA as measured by qRT‐PCR; (F) levels of IL‐6 mRNA as measured by qRT‐PCR; (G) levels of Arg1 mRNA as measured by qRT‐PCR; (H) levels of IL‐10 mRNA as measured by qRT‐PCR. Data are expressed as the mean ± SD ( n = 3 biological replicates). * p < 0.05, ** p < 0.01 vs. sham group; # p < 0.05, ## p < 0.01 vs. model group.

    Article Snippet: The Sox9 (Cat No., BS‐10725R), Collagen II (Cat No., bsm‐33409M), F4/80 (Cat No. 263101), CD86 (Cat No. bs‐1035R), and CD206 (Cat No. bsm‐60761R) antibodies were bought from Bioss (China).

    Techniques: Analysis, Quantitative RT-PCR

    Effect of DID on macrophage polarization and PPAR signaling‐mediated FAO pathway in BMDMs. (A) Representative pictures of immunofluorescence staining (20×, 50 μm) of F4/80 + CD86 + and F4/80 + CD206 + in response to LPS/IFN‐γ co‐stimulation; (B) quantitative analysis of F4/80 + CD86 + and F4/80 + CD206 + ; (C) representative pictures of immunofluorescence staining (20×, 50 μm) of F4/80 + CD86 + and F4/80 + CD206 + in response to IL‐4/IL‐13 co‐stimulation; (D) quantitative analysis of F4/80 + CD86 + and F4/80 + CD206 + ; (E) levels of CoA were detected by ELISA; (F) levels of Cpt1a mRNA as measured by qRT‐PCR; (G) levels of ACS mRNA as measured by qRT‐PCR; (H) levels of PPAR‐γ protein as measured by Western blotting; (I) levels of RXRA protein as measured by Western blotting; (J) protein bands were analyzed by Western blotting for PPAR‐γ and RXRA. Data are expressed as the mean ± SD ( n = 3 biological replicates). * p < 0.05, ** p < 0.01 vs. BMDMs (M0) group; # p < 0.05, ## p < 0.01 vs. BMDMs (M1) group.

    Journal: Immunity, Inflammation and Disease

    Article Title: Didymin may Enhance Tendon‐Bone Healing Partly by Promoting PPAR‐Mediated Fatty Acid Oxidation and Macrophage M2 Polarization

    doi: 10.1002/iid3.70485

    Figure Lengend Snippet: Effect of DID on macrophage polarization and PPAR signaling‐mediated FAO pathway in BMDMs. (A) Representative pictures of immunofluorescence staining (20×, 50 μm) of F4/80 + CD86 + and F4/80 + CD206 + in response to LPS/IFN‐γ co‐stimulation; (B) quantitative analysis of F4/80 + CD86 + and F4/80 + CD206 + ; (C) representative pictures of immunofluorescence staining (20×, 50 μm) of F4/80 + CD86 + and F4/80 + CD206 + in response to IL‐4/IL‐13 co‐stimulation; (D) quantitative analysis of F4/80 + CD86 + and F4/80 + CD206 + ; (E) levels of CoA were detected by ELISA; (F) levels of Cpt1a mRNA as measured by qRT‐PCR; (G) levels of ACS mRNA as measured by qRT‐PCR; (H) levels of PPAR‐γ protein as measured by Western blotting; (I) levels of RXRA protein as measured by Western blotting; (J) protein bands were analyzed by Western blotting for PPAR‐γ and RXRA. Data are expressed as the mean ± SD ( n = 3 biological replicates). * p < 0.05, ** p < 0.01 vs. BMDMs (M0) group; # p < 0.05, ## p < 0.01 vs. BMDMs (M1) group.

    Article Snippet: The Sox9 (Cat No., BS‐10725R), Collagen II (Cat No., bsm‐33409M), F4/80 (Cat No. 263101), CD86 (Cat No. bs‐1035R), and CD206 (Cat No. bsm‐60761R) antibodies were bought from Bioss (China).

    Techniques: Immunofluorescence, Staining, Analysis, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Western Blot

    Validation of PPAR signaling‐mediated FAO pathway involved in DID‐induced macrophage polarization in BMDMs. (A) Levels of PPAR‐γ and RXRA protein as measured by Western blotting; (B) levels of CoA were detected by ELISA; (C) immunofluorescence staining (20×, 50 μm) of F4/80 + CD86 + and F4/80 + CD206 + ; (D) levels of Arg1, IL‐1β, IL‐10, and IL‐6 mRNA as measured by qRT‐PCR. Data are expressed as the mean ± SD ( n = 3 biological replicates). * p < 0.05, ** p < 0.01 vs. BMDMs (M0) group; # p < 0.05, ## p < 0.01 vs. BMDMs (M1) group; & p < 0.05, && p < 0.01 vs. BMDMs (M1) + DID group.

    Journal: Immunity, Inflammation and Disease

    Article Title: Didymin may Enhance Tendon‐Bone Healing Partly by Promoting PPAR‐Mediated Fatty Acid Oxidation and Macrophage M2 Polarization

    doi: 10.1002/iid3.70485

    Figure Lengend Snippet: Validation of PPAR signaling‐mediated FAO pathway involved in DID‐induced macrophage polarization in BMDMs. (A) Levels of PPAR‐γ and RXRA protein as measured by Western blotting; (B) levels of CoA were detected by ELISA; (C) immunofluorescence staining (20×, 50 μm) of F4/80 + CD86 + and F4/80 + CD206 + ; (D) levels of Arg1, IL‐1β, IL‐10, and IL‐6 mRNA as measured by qRT‐PCR. Data are expressed as the mean ± SD ( n = 3 biological replicates). * p < 0.05, ** p < 0.01 vs. BMDMs (M0) group; # p < 0.05, ## p < 0.01 vs. BMDMs (M1) group; & p < 0.05, && p < 0.01 vs. BMDMs (M1) + DID group.

    Article Snippet: The Sox9 (Cat No., BS‐10725R), Collagen II (Cat No., bsm‐33409M), F4/80 (Cat No. 263101), CD86 (Cat No. bs‐1035R), and CD206 (Cat No. bsm‐60761R) antibodies were bought from Bioss (China).

    Techniques: Biomarker Discovery, Western Blot, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Staining, Quantitative RT-PCR

    CD73‐overexpressing ADSCs regulate macrophage polarization toward the M2 and inhibit NLRP3 inflammasome activation. (A) Flow cytometry results showing macrophage polarization in each group. CD86 was detected using FITC‐conjugated antibody, and CD163 was detected using PE‐conjugated antibody. (B) Western blot and quantitative analysis of NLRP3, ASC, and caspase‐1 expression in vitro. (C) Quantitative analysis of Western blot. (D) ELISA analysis of IL‐1β and IL‐18 levels in each group in vitro. Statistical significance is indicated as ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001. All experiments were independently performed in triplicate. The full‐length, uncropped Western blot images are provided in Figure . ELISA data are summarized in Tables and .

    Journal: Stem Cells International

    Article Title: CD73‐Overexpressing ADSCs Promote Neurogenic Bladder Repair via A2A–JAK1/STAT3‐Mediated Modulation of Inflammation and Macrophage Polarization

    doi: 10.1155/sci/8564160

    Figure Lengend Snippet: CD73‐overexpressing ADSCs regulate macrophage polarization toward the M2 and inhibit NLRP3 inflammasome activation. (A) Flow cytometry results showing macrophage polarization in each group. CD86 was detected using FITC‐conjugated antibody, and CD163 was detected using PE‐conjugated antibody. (B) Western blot and quantitative analysis of NLRP3, ASC, and caspase‐1 expression in vitro. (C) Quantitative analysis of Western blot. (D) ELISA analysis of IL‐1β and IL‐18 levels in each group in vitro. Statistical significance is indicated as ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001. All experiments were independently performed in triplicate. The full‐length, uncropped Western blot images are provided in Figure . ELISA data are summarized in Tables and .

    Article Snippet: For intracellular marker staining, cells were blocked with 5% bovine serum albumin and incubated for 30 min at room temperature with FITC‐conjugated antimouse CD86 (Elabscience, Wuhan, Hubei, China; 1:100 dilution) and PE‐conjugated antimouse CD163 antibodies (Elabscience, Wuhan, Hubei, China; 1:100 dilution).

    Techniques: Activation Assay, Flow Cytometry, Western Blot, Analysis, Expressing, In Vitro, Enzyme-linked Immunosorbent Assay

    Representative double immunofluorescence images of CD86 (red) and CD206 (green) (A) magnification: ×400. Quantification of relative fluorescence intensity of CD86 (B) and CD206 (C) Relative fluorescence intensity was calculated as the ratio of the MFI of each sample to the mean MFI of the Con group. MFI: mean fluorescence intensity. * P < 0.05 compared with the Con group.

    Journal: Frontiers in Pediatrics

    Article Title: Trans-cuff budesonide delivery and its effects on endotracheal intubation-induced subglottic injury

    doi: 10.3389/fped.2026.1886065

    Figure Lengend Snippet: Representative double immunofluorescence images of CD86 (red) and CD206 (green) (A) magnification: ×400. Quantification of relative fluorescence intensity of CD86 (B) and CD206 (C) Relative fluorescence intensity was calculated as the ratio of the MFI of each sample to the mean MFI of the Con group. MFI: mean fluorescence intensity. * P < 0.05 compared with the Con group.

    Article Snippet: For double immunofluorescence staining of CD86 and CD206, sections were first incubated overnight at 4 °C with mouse anti-CD86 antibody (68674–2-Ig, Proteintech Group, Inc., Wuhan, China), followed by HRP-conjugated anti-mouse secondary antibody and TSA-570 amplification.

    Techniques: Immunofluorescence, Fluorescence